PCI Genomics

300

Title *

High quality genome assembly and annotation (v1) of the eukaryotic terrestrial microalga *Coccomyxa viridis* SAG 216-4use asterix (*) to get italics

Authors *

Anton Kraege, Edgar Chavarro-Carrero, Nadège Guiglielmoni, Eva Schnell, Joseph Kirangwa, Stefanie Heilmann-Heimbach, Kerstin Becker, Karl Köhrer, Philipp Schiffer, Bart P. H. J. Thomma, Hanna RovenichPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

Year *

2024

Picture *

Abstract *

<p>Unicellular green algae of the genus Coccomyxa are recognized for their worldwide distribution and ecological versatility. Most species described to date live in close association with various host species, such as in lichen associations. However, little is known about the molecular mechanisms that drive such symbiotic lifestyles. We generated a high-quality genome assembly for the lichen photobiont Coccomyxa viridis SAG 216-4 (formerly C. mucigena). Using long-read PacBio HiFi and Oxford Nanopore Technologies in combination with chromatin conformation capture (Hi-C) sequencing, we assembled the genome into 21 scaffolds with a total length of 50.9 Mb, an N50 of 2.7 Mb and a BUSCO score of 98.6%. While 19 scaffolds represent full-length nuclear chromosomes, two additional scaffolds represent the mitochondrial and plastid genomes. Transcriptome-guided gene annotation resulted in the identification of 13,557 protein-coding genes, of which 68% have annotated PFAM domains and 962 are predicted to be secreted. </p>

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Keywords (optional)

Coccomyxa viridis, EBP, ERGA, long-read sequencing, genome assembly, genome annotation, Trebouxiophyceae

Methods that require specific expertise (optional)

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Thematic fields *

ERGA Pilot

Suggested reviewers - Suggest up to 10 reviewers (provide names and Email addresses). (Optional)

Fabian Haas suggested: Rory James Craig <rory.craig@berkeley.edu> No need for them to be recommenders of PCI Genomics. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

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Submission date

2023-11-09 11:54:43

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Iker Irisarri

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