PCI Genomics

241

Title *

Near-chromosome level genome assembly of devil firefish, *Pterois miles*use asterix (*) to get italics

Authors *

Christos V. Kitsoulis, Vasileios Papadogiannis, Jon B. Kristoffersen, Elisavet Kaitetzidou, Aspasia Sterioti, Costas S. Tsigenopoulos, Tereza ManousakiPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

Year *

2023

Picture *

Abstract *

Devil firefish (Pterois miles), a member of Scorpaenidae family, is one of the most successful marine non-native species, dominating around the world, that was rapidly spread into the Mediterranean Sea, through the Suez Canal, originating from the Indian Ocean. Even though lionfishes (Scorpaenidae) are identified among the most prosperous marine invaders, within this taxonomic group, the genomic resources are scant, while reference genome assemblies are totally absent. Here, we built and analyzed the first reference genome assembly of P. miles and explored its evolutionary background. The resulting genome assembly consisted of 660 contigs and scaffolds (N50 = 14,5 Mb) with a total size of about 902 Mb, while delivering 98% BUSCO completeness. We identified and described the large amount of transposable elements present in the genome and based on genomic data we constructed the first teleost phylogeny which includes a member of genus Pterois. The high-quality and contiguity de novo genome assembly built herein provides a valuable resource for future studies in species’ biology and ecology, lionfish phylogeny, the influence of transposable elements on the evolution of vertebrate genomes and fish toxins evolution.

Indicate the full web address (DOI or URL) giving public access to these data (if you have any problems with the deposit of your data, please contact contact@genomics.peercommunityin.org). In case all raw data are included in the preprint, indicate the DOI or URL of the preprint. *

https://www.ebi.ac.uk/ena/browser/view/ERR10286272, https://www.ebi.ac.uk/ena/browser/view/ERR10286273, https://www.ebi.ac.uk/ena/browser/view/CAMQGK010000000You should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

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https://github.com/ckitsoulis/Pterois-miles-Genome, https://github.com/ckitsoulis/ELDARYou should fill this box only if you chose 'Scripts were used to obtain or analyze the results'. URL must start with http:// or https://

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Keywords (optional)

lionfish, genome assembly, Scorpaenidae, phylogenomic analysis

Methods that require specific expertise (optional)

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Thematic fields *

Evolutionary genomics

Suggested reviewers - Suggest up to 10 reviewers (provide names and Email addresses). (Optional)

Luca Bargelloni (luca.bargelloni@unipd.it), Giacomo Bernardi (bernardi@ucsc.edu)

e.g. John Doe john@doe.com

No need for them to be recommenders of PCI Genomics. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

Opposed reviewers - Suggest up to 5 people not to invite as reviewers. (Optional)

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Submission date

2023-01-17 12:37:20

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Iker Irisarri

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