Genetic bases of resistance to the rice hoja blanca disease deciphered by a QTL approachuse asterix (*) to get italics

Alexander Silva, Maria Elker Montoya, Constanza Quintero, Juan Cuasquer, Joe Tohme, Eduardo Graterol, Maribel Cruz, Mathias LorieuxPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

2023

<p style="text-align: justify;">Rice hoja blanca (RHB) is one of the most serious diseases in rice growing areas in tropical Americas. Its causal agent is Rice hoja blanca virus (RHBV), transmitted by the planthopper <em>Tagosodes orizicolus </em>Muir. Genetic resistance is the most effective and environment-friendly way of controlling the disease. So far, only one major quantitative trait locus (QTL) of <em>Oryza sativa </em>ssp. japonica origin, qHBV4.1, that alters incidence of the virus symptoms in two Colombian cultivars has been reported. This resistance has already started to be broken, stressing the urgent need for diversifying the resistance sources. In the present study we performed a search for new QTLs of O. sativa indica origin associated with RHB resistance. We used four F2:3 segregating populations derived from indica-resistant varieties crossed with a highly susceptible japonica pivot parent. Beside the standard method for measuring disease incidence, we developed a new method based on computer-assisted image processing to determine the affected leaf area (ALA) as a measure of symptoms severity. Based on the disease severity and incidence scores in the F3 families under greenhouse conditions, and SNP genotyping of the F2 individuals, we identified four new indica QTLs for RHB resistance on rice chromosomes 4, 6 and 11, namely qHBV4.2WAS208, qHBV6.1PTB25, qHBV11.1 and qHBV11.2. We also confirmed the wide-range action of qHBV4.1. Among the five QTLs, qHBV4.1 and qHBV11.1 had the largest effects on incidence and severity, respectively. These results provide a more complete understanding of the genetic bases of RHBV resistance in the cultivated rice gene pool, and can be used to develop marker-aided breeding strategies to improve RHB resistance. The power of joint- and meta- analyses allowed precise mapping and candidate genes identification, providing the basis for positional cloning of the two major QTLs qHBV4.1 and qHBV11.1.</p>

https://doi.org/10.23708/EERVFWYou should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

ORYZA SATIVA, RICE HOJA BLANCA DISEASE, CANDIDATE GENES, QTL

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Functional genomics, Plants

Ken McNally - K.McNally@irri.org, Baboucarr Manneh - B.Manneh@cgiar.org, Laurence Albar - laurence.albar@ird.fr

e.g. John Doe john@doe.com

No need for them to be recommenders of PCI Genomics. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

2022-11-09 09:13:30

Olivier Panaud