PCI Genomics

156

Title

Chromosome-level genome assembly and annotation of two lineages of the ant Cataglyphis hispanica: steppingstones towards genomic studies of hybridogenesis and thermal adaptation in desert antsuse asterix (*) to get italics

Authors

Hugo Darras, Natalia de Souza Araujo, Lyam Baudry, Nadège Guiglielmoni, Pedro Lorite, Martial Marbouty, Fernando Rodriguez, Irina Arkhipova, Romain Koszul, Jean-François Flot, Serge AronPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

Year

2022

Picture

Abstract

Cataglyphis are thermophilic ants that forage during the day when temperatures are highest and sometimes close to their critical thermal limit. Several Cataglyphis species have evolved unusual reproductive systems such as facultative queen parthenogenesis or social hybridogenesis, which have not yet been investigated in detail at the molecular level. We generated high-quality genome assemblies for two hybridogenetic lineages of the Iberian ant Cataglyphis hispanica using long-read Nanopore sequencing and exploited chromosome conformation capture (3C) sequencing to assemble contigs into 26 and 27 chromosomes, respectively. Further karyotype analyses confirm this difference in chromosome numbers between lineages; however, they also suggest it may not be fixed among lineages. We obtained transcriptomic data to assist gene annotation and built custom repeat libraries for each of the two assemblies. Comparative analyses with 19 other published ant genomes were also conducted. These new genomic resources pave the way for exploring the genetic mechanisms underlying the remarkable thermal adaptation and the molecular mechanisms associated with transitions between different genetic systems characteristic of the ant genus Cataglyphis.

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https://www.ncbi.nlm.nih.gov/sra/SRR17481978, https://www.ncbi.nlm.nih.gov/sra/SRR17481992, https://www.ncbi.nlm.nih.gov/nuccore/JAJUXC000000000.1/, https://www.ncbi.nlm.nih.gov/nuccore/JAJUXE000000000.1/You should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

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https://doi.org/10.6084/m9.figshare.17964695.v7You should fill this box only if you chose 'Codes have been used in this study'. URL must start with http:// or https://

Keywords

Chromosome-level genome assembly

Methods that require specific expertise (optional)

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Thematic fields

Evolutionary genomics

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Submission date

2022-01-13 16:47:30

Recommender

Nadia Ponts

Reviewers

Nicolas Nègre, Isabel Almudi

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